Fig. 2.
Teneligliptin effects on HG stress markers: ROS, NAD(P)H oxidase subunits and TXNIP in HUVECs cultured under HG/HM conditions. HUVECs were maintained under NG, HG or HM conditions during 21 days. During the exposure, teneligliptin was added chronically to the medium at 0.1, 1.0 or 3.0 μmol/L. We used as control the DPP-4 inhibitor sitagliptin at the concentration of 0.5 μmol/L. a ROS production was stained by 20 μM H2DCFDA for 30 min, and its oxidation product (DCF) fluorescence indicated ROS formation. b,c Total cellular RNA was isolated from HUVECs and mRNAs encoding for NOX4, P22 −phox and TXNIP genes were assessed by qRT-PCR and expressed relative to ACTB. *p < 0.05 and **p < 0.01 vs. NG. #p < 0.05 vs. HG. $p < 0.05 vs. HM. Bars represent mean ± SEM for six independent experiments