Fig. 5.

Teneligliptin effects on ER function in HUVECs cultured under HG/HM conditions. HUVECs were maintained under NG, HG or HM conditions during 21 days. During the exposure, teneligliptin was added chronically to the medium at 0.1, 1.0 or 3.0 μmol/L. We used as control the DPP-4 inhibitor sitagliptin at the concentration of 0.5 μmol/L. Total cellular RNA was isolated from HUVECs and mRNAs encoding for the different ER stress markers were assessed by qRT-PCR and expressed relative to ACTB. *p < 0.05 and vs. NG. #p < 0.5 vs. HG. $p < 0.5 vs. HM. Bars represent mean ± SEM for six independent experiments