Table 6.
RTqPCR differential expression analysis results using samples from the validation cohort of microbial keratitis patients.
| Category | Gene | BK vs. C | FK vs. C | BK vs. FK | |||
|---|---|---|---|---|---|---|---|
| FC | FDR-adjusted p-value | FC | FDR-adjusted p-value | FC | FDR-adjusted p-value | ||
| Immune response | CXCL5 | 2792.89 | 3.21E-16 | 1564.99 | 9.69E-21 | 1.78 | 0.947 |
| CXCL6 | 205.85 | 7.50E-06 | 196.07 | 3.68E-08 | 1.05 | 0.957 | |
| CCL20 | 1320.91 | 3.78E-16 | 656.98 | 8.32E-20 | 2.01 | 0.947 | |
| CCL3L1 | 147.23 | 1.95E-07 | 180.04 | 2.92E-11 | 0.82 | 0.947 | |
| CXCL1 | 109.8 | 2.93E-11 | 84.25 | 8.61E-15 | 1.3 | 0.947 | |
| CXCL8 | 1035.87 | 2.15E-14 | 1272.22 | 2.15E-21 | 0.81 | 0.947 | |
| IL1B | 959.19 | 1.44E-13 | 1296.59 | 1.32E-20 | 0.74 | 0.947 | |
| TNF | 65.29 | 1.98E-06 | 74.92 | 1.36E-09 | 0.87 | 0.947 | |
| IL17A | 201.64 | 1.18E-04 | 218.43 | 1.27E-06 | 0.92 | 0.957 | |
| IL23A | 1238.8 | 3.84E-14 | 1665.71 | 2.15E-21 | 0.74 | 0.947 | |
| IFNG | 138.09 | 8.47E-04 | 129.84 | 4.11E-05 | 1.06 | 0.957 | |
| IL18 | 27.5 | 2.84E-05 | 19.34 | 2.76E-06 | 1.42 | 0.947 | |
| IL12B | 190.28 | 3.71E-05 | 182.04 | 3.73E-07 | 1.05 | 0.957 | |
| TREM1 | 206.05 | 8.59E-10 | 423.63 | 4.60E-17 | 0.49 | 0.947 | |
| CYTL1 | 0.05 | 6.46E-03 | 0.03 | 1.32E-04 | 1.46 | 0.947 | |
| AQP9 | 1167.59 | 1.29E-13 | 1591.01 | 9.69E-21 | 0.73 | 0.947 | |
| PRRs | CLEC4A | 135.1 | 1.03E-09 | 311.48 | 1.20E-17 | 0.43 | 0.947 |
| CLEC7A | 19.42 | 1.26E-04 | 24.77 | 3.23E-07 | 0.78 | 0.947 | |
| FCER1G | 77.22 | 4.24E-09 | 145.47 | 4.32E-16 | 0.53 | 0.947 | |
| FCN1 | 268.45 | 5.35E-10 | 465.36 | 1.40E-16 | 0.58 | 0.947 | |
| FPR1 | 161.68 | 1.22E-10 | 253.77 | 2.15E-17 | 0.64 | 0.947 | |
| SCARA3 | 1.64 | 6.16E-01 | 0.93 | 9.28E-01 | 1.76 | 0.947 | |
| TLR2 | 270.16 | 1.68E-12 | 497.91 | 9.69E-21 | 0.54 | 0.947 | |
| TLR4 | 76.09 | 7.50E-06 | 106.71 | 2.72E-09 | 0.71 | 0.947 | |
| MARCO | 398.21 | 1.38E-11 | 251.09 | 9.53E-15 | 1.59 | 0.947 | |
| NLRP3 | 35.68 | 3.72E-05 | 48.95 | 3.22E-08 | 0.73 | 0.947 | |
| ECM | COL1A1 | 6.66 | 2.91E-02 | 3.21 | 9.56E-02 | 2.07 | 0.947 |
| COL5A1 | 61.06 | 5.24E-05 | 47.83 | 2.20E-06 | 1.28 | 0.947 | |
| KERA | 0.03 | 1.28E-02 | 0.04 | 3.57E-03 | 0.8 | 0.947 | |
| KRT6B | 2101.97 | 6.00E-21 | 2937.8 | 3.19E-31 | 0.72 | 0.947 | |
| ALDH1A1 | 1.8 | 5.27E-01 | 0.74 | 6.80E-01 | 2.44 | 0.947 | |
| MMPs | MMP1 | 536.49 | 1.39E-12 | 565.54 | 3.04E-18 | 0.95 | 0.957 |
| MMP10 | 459.9 | 2.93E-11 | 405.57 | 1.17E-15 | 1.13 | 0.947 | |
| MMP12 | 2791.89 | 1.07E-08 | 717.96 | 3.14E-09 | 3.89 | 0.947 | |
| MMP28 | 12.71 | 1.70E-02 | 8.39 | 1.28E-02 | 1.52 | 0.947 | |
| MMP7 | 335.79 | 7.50E-09 | 296.06 | 3.26E-12 | 1.13 | 0.947 | |
| MMP9 | 7754.12 | 5.23E-22 | 11661.09 | 8.82E-33 | 0.66 | 0.947 | |
| ADAM19 | 156.56 | 7.64E-08 | 284.19 | 2.46E-13 | 0.55 | 0.947 | |
| Anti-microbial peptides | PI3 | 4787.64 | 6.43E-23 | 5666.71 | 8.82E-33 | 0.84 | 0.947 |
| S100A7 | 7119.6 | 1.44E-13 | 3891.02 | 1.90E-17 | 1.83 | 0.947 | |
| S100A9 | 831.43 | 6.43E-23 | 921.41 | 8.82E-33 | 0.9 | 0.947 | |
| DEFB4B | 1421.62 | 6.51E-14 | 1208.72 | 2.57E-19 | 1.18 | 0.947 | |
| Peptidase inhibitors | SERPINA1 | 134.64 | 1.29E-10 | 311.83 | 2.57E-19 | 0.43 | 0.947 |
| TIMP1 | 3.81 | 1.23E-02 | 4.4 | 5.26E-04 | 0.87 | 0.947 | |
| TIMP3 | 1.09 | 9.26E-01 | 0.59 | 4.97E-01 | 1.84 | 0.947 | |
BK, Bacterial Keratitis; FK, Fungal keratitis; C, Controls; PRRs, Pattern Recognition Receptors; ECM, Extra-Cellular Matrix; MMPs, Matrix Metallopeptidases.