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. 2017 Jun;23(6):899–909. doi: 10.1261/rna.056788.116

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© 2017 Rihan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 6. — SMN colocalizes with the FL-wt and G-motif + 3′UTR region of Anxa2 mRNA. (A) Endogenous SMN protein (green spots) colocalizes with the full-length Anxa2 mRNA (FL-wt) and with the G-motif + 3′UTR containing reporter mRNAs (red spots). Differentiated NSC-34 cells were fixed and processed for FISH using MS2 antisense probes and for IF with anti-SMN antibodies. Arrowheads indicate colocalizing spots. Scale bar, 10 µm. Insets on the bottom of each panel are enlarged images of boxed sections. (B) Quantification of the percentage of mRNA spots colocalizing with SMN spots. Mean and SEM are shown. Statistical significance was calculated using the Student t-test (ns, not significant; n = 24, n corresponds to the number of cells that were analyzed).