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. 2017 Jun;23(6):910–926. doi: 10.1261/rna.060640.117

FIGURE 5.

FIGURE 5.

Replication-dependent histone mRNAs accumulate in Skiv2l2 knockdown cells. (A) Transcripts up-regulated in P19 cells following Skiv2l2 knockdown as measured by RNA-seq. Transcripts up-regulated in Skiv2l2 knockdown cells were categorized based on coding and noncoding annotations (q-value <0.05, >1.5-fold change). (B) Transcripts down-regulated in P19 cells following Skiv2l2 knockdown as measured by RNA-seq. Transcripts down-regulated in Skiv2l2 knockdown cells categorized based on coding and noncoding annotations (q-value <0.05, >1.5-fold change). (C) qRT-PCR of histone mRNAs in N2A cells. Histone mRNA levels were calculated using ΔCq values and normalized to β-actin mRNA (error bars represent ±SD for n = 3). (D) qRT-PCR of histone mRNAs in P19 cells. Histone mRNA levels calculated as in C (error bars represent ±SD for n = 3).