Figure 6.

MCM8–9 promotes DNA synthesis in DSB-induced HR. (A) Schematic illustration showing the generation of STGC and LTGC from the SCneo substrate integrated at the ovalbumin locus in the chicken DT40 genome. The expressed I-SceI endonuclease cleaves the I-SceI site in S2neo and induces HR repair between sister chromatids. STGC is generated by copying 0.3–0.4 kb from 3′ neo, while LTGC is produced by a extensive DNA synthesis (>3.3 kb) from 3′ neo. (B) After I-SceI expression in wild-type, MCM8-KO, and MCM9-KO DT40 cells, the recombination frequency was calculated by counting G418-resistant colonies. Error bars indicate standard deviation. n = 3. (C) Southern blotting using a probe containing 3′ neo. Arrows indicate the positions of the STGC and LTGC products. (D) Frequency of each gene conversion event. Clones that were unable to be classified into either STGC or LTGC are shown as “aberrant.”