Figure 2.

TDG is enriched at enhancers and promoters genome-wide and colocalizes with the XPC complex subunit RAD23B. (A) The number of ChIP-seq peaks identified using MACS2 for each data set. Approximately 93% of biotin–TDG (Neri et al. 2015) ChIP-seq peaks overlap with RAD23B in mouse ESCs. (B) Heat map of TDG- and RAD23B-bound regions over a ±5-kb window around the TSSs of genes. Genes in each heat map are sorted according to descending levels of TDG ChIP-seq signal. (C) Integrative Genomics Viewer (IGV)-computed ChIP-seq tracks are plotted as (number of reads) × [1,000,000/(total read count)] for pluripotency genes Nanog and Pou5f1. Mock and normal IgG were used as specificity controls for the TDG and RAD23B ChIP, respectively.