Figure 6.

XPC increases somatic reprogramming fidelity and iPSC self-renewal capacity. (A) Schematic diagram for human iPSC induction modified from methods in Okita et al. (2011). (B) Flow cytometry analysis of control and XPC complex (XPC–RAD23B–CETN2) gain-of-function cell populations 24 d post-induction for the late stage human iPSC marker TRA-1-60. (C) The average number of colonies obtained in the reprogramming experiment depicted in B. (D) iPSCs derived from control or XPC–RAD23B–CETN2-overexpressing HDFs were challenged with single-cell dissociation and allowed to recover for 3–4 d before staining with alkaline phosphatase (AP). The average number of AP⁺ colonies formed per 2.5 × 10⁴ single cells plated. Error bars depict the standard deviation. n = 3. (*) P < 0.05, calculated by two-tailed Student's t-test.