Table 1. Stabilization effects of different ligands on Tel22 G-quadruplex DNA, i-motif DNA and dsDNA^a.

	Δ T m (°C)
Ligand	G-quadruplex	i-motif	dsDNA
LΔ1a	0.2 ± 0.3	−0.1 ± 0.2	0.3 ± 0.1
LΛ1a	0.1 ± 0.1	0.1 ± 0.4	0.4 ± 0.2
Δ1a	10.8 ± 0.3	0.2 ± 0.3	0.3 ± 0.2
Λ1a	0.2 ± 0.2	0.1 ± 0.1	0.3 ± 0.4

^aThe dsDNA is duplex DNA formed by complementary sequence of Tel22 G-quadruplex DNA and i-motif DNA. L_Δ1a and L_Λ1a are constituent ligands that used for synthesizing Δ1a and Λ1a. Melting assays were carried out in 10 mM Tris buffer containing 10 mM KCl at pH 7.2 or pH 5.5 (only for i-motif DNA). DNA is 3 μM in strand. The concentration of the Ligands is equivalent with corresponding DNA. The values were the average of three independent measurements.