Table 3. Binding stoichiometry and thermodynamic parameters for the interaction of Tel22 DNA with Δ1a and Λ1aa.
Δ1a | Λ1a | |
---|---|---|
n | 0.5 ± 0.1 | 0.6 ± 0.2 |
ΔG025 (kcal mol −1) | −9.4 ± 0.4 | −7.2 ± 0.5 |
ΔH0 (kcal mol −1) | −15.4 ± 1.4 | −11.8 ± 0.7 |
TΔS0 (kcal mol−1 K −1) | −6.0 ± 0.4 | −4.6 ± 0.5 |
aAll data were derived from ITC experiments. ΔH0 and n (stoichiometry) was directly obtained from ITC. ΔG025 was obtained from the relation ΔG0 = -RTlnKa (Ka was listed in Table 1). TΔS0 obtained from the relation TΔS0 = ΔH0-ΔG0.