Table 4. Stabilization effect of Δ1a and Λ1a on different telomeric G-quadruplex DNA in K+ containing buffera.
Telomeric DNA | Structure | DNA Tm (°C) | +Δ1a ΔTm (°C) | +Λ1a ΔTm (°C) |
---|---|---|---|---|
Human (Tel22) | Hybrid | 54.6 ± 0.3 | 10.8 ± 0.3 | 0.2 ± 0.2 |
Oxytrichab | Antiparallel | 61.6 ± 0.2 | −5.0 ± 0.5 | −4.7 ± 0.4 |
Tetrahymenab | Hybrid | 58.2 ± 0.1 | 0.7 ± 0.3 | 0.8 ± 0.3 |
Tel22-TTT | Hybrid | 64.8 ± 0.3 | 0.2 ± 0.3 | 0.4 ± 0.2 |
aMelting assays were carried out in 10 mM Tris buffer containing 10 mM KCl at pH 7.2. DNA is 3 μM in strand. The concentration of the Ligands is equivalent with corresponding DNA. The values were the average of three independent measurements.
bThe Tm of the was measured in 1 mM K+ containing buffer.