Figure 7.

Analysis of partially processed transcripts containing mt-N4l/4. (A) Circularized RNA sequencing of wild-type mouse mitochondrial RNA. Reads mapping between mt-Nd4l/4 and other mitochondrial RNAs are illustrated by lines between the 5΄ and 3΄ nucleotide positions. The locations of genes are shown in a schematic, where heavy strand genes are shown in the outer ring and light strand genes in the inner ring. Genes encoding rRNAs are shown in green, mRNAs are shown in light blue, tRNAs in grey, the D-loop in white, and the mRNA of interest, mt-Nd4l/4, is shown in orange. (B) Circularized RNA sequencing of mitochondrial RNA from Mrpp3 knock out mice. (C) PARE data illustrating the various positions of mt-Nd4l/4's short 5΄-UTRs. (D) Northern blotting of heart RNA from wild-type and Mrpp3 knock out mice using a probe specific for mt-Nd4l/4.