Figure 4.

(A) Lowest energy conformers obtained from XPLOR refinement of idealized B-form DNA using experimental distance constraints and RDCs (see ‘Materials and Methods’ section) for A₆-DNA (red, PDB ID: 5UZF, BMRB ID: 30254) and A₆-DNA^m1A16 (blue, PDB ID: 5UZI, BMRB ID: 30255). The single m¹A•T HG bp is in gray for A₆-DNA^m1A16. (B) Cartoon representation of A₆-DNA (red), A₆-DNA^m1A16 (blue), DNA-p53 (cyan) and MATα2-homeodomain (magenta) complexes depicting major-groove kinking at the HG bp. An idealized B-form DNA helix (in gray) is overlaid for reference (HG bps shown in orange). (C) Local kink angle (β_h) as a function of the junction position and global bending calculated for A₂-DNA (green), A₆-DNA (red) and A₆-DNA^m1A16 (blue).