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. 2017 Feb 3;45(9):5086–5099. doi: 10.1093/nar/gkx075

Figure 4.

Figure 4.

Effect of knocking down p27 or PCAF on the expression of target genes. (A) HCT116 cells were infected with a specific shRNA for p27 (shp27) or with a control shRNA (sh(–)). Then, mRNA levels of different common target genes (see Table 1) were determined by qPCR. In these cells, the levels of primary non spliced transcripts (PNST) were also determined by qPCR (B). (C) HCT116 cells were infected with a specific shRNA for PCAF (shPCAF) or with a control shRNA (sh(–)) and the levels of mRNA of different common target genes were subsequently determined by qPCR. In these cells, the levels of primary non spliced transcripts (PNST) were also determined by qPCR (D). Levels of mRNA (E) or of primary non spliced transcripts (PNST) (F) from different common target genes were determined in p27 knocked down cells using the CRISPR/Cas9 (CR p27) methodology and in control cells (CR Ctrl). Levels of mRNA (G) or of primary non spliced transcripts (PNST) (H) from different common target genes were determined in PACF knocked down cells using the CRISPR/Cas9 (CR PCAF) methodology and in control cells (CR Ctrl). In all experiments results are the mean value ± SEM of four independent experiments and are represented as fold enrichment versus control. Statistical analyses were performed using the t-student's test. *P < 0.05, **P < 0.01 and ***P < 0.001.