Figure 5.

Effect of knocking down both, p27 and PCAF on the expression of target genes. (A) HCT116 cells were infected with a specific shRNA for p27 alone, or with shRNAs for p27 and PCAF or with a control shRNA (sh(–)). Then, the levels of primary non spliced transcripts (PNST) were determined by qPCR. (B). Cells were Knocked down for p27 and PCAF (double knockout) (2KD) using the CRISPR/Cas9 methodology. Then, the levels of PCAF and p27 were determined by WB. Levels of mRNA (C) or of primary non spliced transcripts (PNST) (D) from different common target genes were determined in double knock down cells (CR 2KD) and in control cells (CR Ctrl) by qPCR. In all experiments results are the mean value ± SEM of four independent experiments and are represented as fold enrichment versus control. Statistical analyses were performed using the t-student's test. *P < 0.05, **P < 0.01 and ***P < 0.001.