Figure 4.

VELUCT knockdown was not detectable with siRNAs and siPOOLs, but with ASOs. H460 cells were transfected with 30 nM VELUCT-specific siRNAs (A) or siPOOLs (B), 10 nM HDAC4-specific siRNAs or siPOOLs (C) or 30 nM VELUCT-specific ASOs (D) for 24 h. VELUCT RNA levels were analyzed in the chromatin fraction using three independent qPCR amplicons. HDAC4 levels were determined in all three subcellular fractions. All RNA levels were normalized to cyclophilin A and relative to the respective NC. Bars show mean ± SD (n = 3–4). *P < 0.05, **P < 0.01, ***P < 0.001.