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. 2017 Mar 15;45(9):5269–5284. doi: 10.1093/nar/gkx176

Table 1. S. pombe strains used in this work.

Strains Genotypes
EM28 h−rgf1::nat, leu1-32, ura4D18
PG244b h−leu1-32, ura4d18
VT14 h−rgf1::his3+, leu1-32, ade6M210, ura4D18, his3d1
SM306c h+rad3::ura4+, ura4D18, leu1-32
EM656 h−rad3::ura4+, rad52:YFP:kan, leu1-32
SM305c h−cds1::ura4+, leu1-32, ura4D18
SM304c h−chk1::ura4+, leu1-32, ura4D18
SM331 h−chk1::ura4+, rgf1::his3+, leu1-32
EM318 h−chk1::ura4+, rad52:YFP:kan, leu1-32
EM672 h−crb2::kanMX6, rad52:YFP:kan, leu1-32, ura4D18
SM429a h−chk1-HA:ura+, leu1-32 ade6-216
SM435 h−rgf1::nat, chk1-HA:ura, leu1-32
SM438d h−atb2-GFP:ura4+, Htt1-RFP: kanMX6, leu1-32
SM439 h−atb2-GFP:ura4+, Htt1-RFP: kanMX6, rgf1::nat, leu1-32
SM329c h−cds1-2HA6His:ura+, leu1-32
SM55 h−rgf1::his3+, leu1-32::rgf1-DEPΔ:leu1+, his3d1, ura4D18, ade6M210
SM63 h−rgf1::his3+, leu1-32::rgf1-NΔ:leu1+, his3d1, ura4D18, ade6M210
SM65 h−rgf1::his3+, leu1-32::rgf1-PHΔ:leu1+, his3d1, ura4D18, ade6M210
SM128 h−rgf1::his3+, leu1-32::rgf1-CNH1Δ:leu1+, his3d1, ura4D18, ade6M210
PG52 h−rgf1::his3+, leu1-32:: rgf1+ (PTTRΔ):leu1+, his3d1, ura4D18, ade6M210
EM4 h−rgf2::kanMx4, leu1-32, ade6M210, ura4D18, his3d1
GI1 h+ehs2-1 (rgf3), leu1-32
MS191 h−pck1::ura4+, leu1-32, ura4D18
PG259b h+pck2::kanMX6, leu1-32
PG347e h−pmk1::ura4+ ura4D18
EM312b h+rho1-596:nat, ura4D18, leu1-32
EM317 h−rho1-596:nat, rad52-YFP:kan, ura4D18, leu1-32
SM308c h−rad52-YFP:kan, leu1-32, ura4D18
SM324 h−rad52-YFP:kan, rgf1::his3+, leu1-32, ura4D18
EM250f h−leu1-32, ura4::RDUX200(kan+)
EM303 h−rgf1::nat, leu1-32, ura4::RDUX200(kan+)
EM350 h−rho1-596:nat, ura4::RDUX200(Kan+) leu1-32
EM309 h−lig4::kan, ura4D18, leu1-32
EM372 h−lig4::kan, rgf1::nat, ura4D18, leu1-32
EM533j h+rad32::ura4+ (rad32 = mre11), ade6-M210 leu1-32 ura4-D18
EM591 h−rad32::ura4+ (rad32 = mre11), rad52:YFP:kan, leu1-32
EM144b h+rad51::kanMX6, ura4D18, leu1-32
EM551 h−rad51::nat, rad52:YFP:kan, ura4::RDUX200(kan+), leu1-32
EM173 h+rad51::kanMX6, rgf1::nat, ura4D18, leu1-32
EM113b h+rad54::kanMX6, ura4D18, leu1-32
EM120 h−rad54::kanMX6, rgf1::nat, ura4D18, leu1-32
EM561 h−rad54::kanMX6, rad52:YFP:kan, ura4-D18, leu1-32
EM117g h−rad54-GFP:hphMX6
EM125 h−rad54-GFP:hphMX6, rgf1::nat
EM625h h−mus81::kanMX6, ura4-D18, ade6-M210
EM635 h−mus81::kanMX6, rad52:YFP:kan
EM142b h+rad52::kanMX6, ura4D18, leu1-32
EM170 h+rad52::kanMX6, rgf1::nat, ura4D18, leu1-32
EM111i h+rad9:YFP:ura4+, rad52:CFP:kan, ura4D18
EM650 h+rad9:YFP:ura4+
EM652 h+rad9:YFP:ura4+, rgf1::nat
EM631h h−rad11A-ts, ura4-D18, leu1-32, ade6-M216
EM644 h−rad11A-ts, rgf1::nat, ura4-D18, leu1-32, ade6-M216
EM658 h−rad11A-ts, rad52:YFP:kan, ura4-D18, leu1-32
EM643k h+rad11-GFP:KanR, leu1-32, ade6M210, ura4D18
EM644 h+rad11-GFP:KanR, rgf1::nat, leu1-32, ade6M210, ura4D18
EM34 h- rgf1Δ-45:ura4+, leu1-32, ura4-D18, his3-D1, ade6-M210
EM44 h+rgf1Δ-45, rgf2::kanMX6, ura4-D18, his3-D1, ade6-M210 (after FOA)
EM697 h−rho1-596:nat, rad52-YFP:kan, rgf1::kanMX6, ura4D18, leu1-32
EM699 h−rho1-596:nat, rgf1::kanMX6, ura4D18, leu1-32
EM718 h−pck1::ura4+rgf1::nat, leu1-32, ura4D18
PG249 h+pck2::kanMX6, rgf1::kanMX6
PG335 h−pmk1::ura4+, rgf1::kanMX6, ura4d18
EM701 h−lig4::kan, rgf1::nat, rad51::kan, ura4D18, leu1-32

All strains were generated in this study except for strains with labela obtained from P. Sunnerhagen (University of Gothenburg), labelb from P. Perez (IBFG, University of Salamanca), labelc from A. Bueno (CIC, University of Salamanca), labeld from J.C. Ribas (IBFG, University of Salamanca), labele from J. Cansado (University of Murcia), labelf from K. Komatsu (Radiation Biology Center, Kyoto University), labelg from T. Nakagawa (Osaka University), labelh from S. Forsburg (University of Southern California), labeli from P. Meister and S. Gasser (FMI, University of Basel), labelj from T. Nakamura (YGRC Japan), labelk from T.C. Humphrey University of Oxford.