Figure 5.

Influence of different Sac3 regions on growth. (A) Serial dilutions of sac3 NULL cells carrying plasmid-borne SAC3 or different SAC3 truncations. Cells were grown at 37°C for 3 days. Although deletion of resides 1–90 (sac3Δ90) grew similarly to SAC3 cells, deletion of the extended loop (sac3Δ125) produced a growth defect similar to sac3 NULL cells. (B) Lag times for Saccharomyces cerevisiae cells transferred from either glucose or raffinose to galactose medium. Cells previously grown in glucose showed longer lag times than cells previously grown in raffinose. SAC3, sac3Δ90 and sac3Δ125 showed similar lag phases, whereas sac3 NULL cells and sac3Δ250 cells showed longer lag times (P < 0.05). Bars represent SDs from six determinations.