Figure 6.

A temperature-dependent experiment of telomere RNA G-quadruplex in molecular crowding conditions. NMR spectra were recorded in crowded solution simulated by 40% (v/v) and 50% (v/v) of ACN. Condition: 0.5 mM RNA in 50 mM KCl and 10 mM Tris-HCl buffer (pH 7.0). The sample is kept for 10 min at each temperature. Red and green spots indicated the dimeric G-quadruplex and two-subunits stacked G-quadruplex. The peaks of single strand are marked with black spots. Temperatures indicated on the right.