Table 2. T _m for higher-order G-quadruplex in dilute and different crowded solutions.

Conditions	T m (°C)	ΔTm (°C)
Dilute solution	52.4	−
40% ACN	58.6	6.2
50% ACN	69.5	17.1
40% DMSO	61.5	9.1
50% DMSO	76.2	23.8
40% PEG 200	56.8	4.4
50% PEG 200	61.9	9.5
40% EtOH	55.0	2.6

^aThe experimental errors for T_m were ±0.5°C.

3 mM RNA in diluted solution and 0.5 mM RNA in molecular crowding solution.