Figure 2.

Expression of Wnt pathway modulators and target genes in WT and TIEG1 KO calvarial osteoblasts. Three independent WT and TIEG1 KO calvarial osteoblast cell lines were differentiated in culture for indicated number of days. Total RNA was isolated and RT-PCR was performed for inhibitors of Wnt signaling (A), transcriptional mediators of Wnt signaling (B) and Wnt target genes (C). (D) Representative images of alizarin red stained WT and TIEG1 KO calvarial osteoblasts prior to and following differentiation. * denotes significant differences (P < 0.05) between WT and TIEG1 KO cells at a given time point.