Table 1. Primer sets used in qRT-PCR and ChIP assays.
| Primer, 5΄ - 3΄ | ||
|---|---|---|
| Gene | Forward | Reverse |
| mWnt1 | CGCTTCCTCATGAACCTTCAC | TGGCGCATCTCAGAGAACAC |
| mWnt2 | TGGCTGAGTGGACTGCAGAGT | GTTGCAGTTCCAGCGATGCT |
| mWnt2b | CCAGACATCATGCGCTCAGTA | GGAACTGGTGCTGGCACTCT |
| mWnt3 | ACCGAGAGGGACCTGGTCTAC | TGGGTTGGGCTCACAAAAGT |
| mWnt3a | GAGACCCTGAGGCCACGTTAC | AGTAGACCAGGTCGCGTTCTG |
| mWnt4 | GTACCTGGCCAAGCTGTCATC | TTTCTCGCACGTCTCCTCTTC |
| mWnt5a | CCACGCTAAGGGTTCCTATGAG | ACGGCCTGCTTCATTGTTGT |
| mWnt5b | GCACCGTGGACAACACATCT | GGCAGTCTCTCGGCTACCTATC |
| mWnt6 | TTATGGATGCGCAGCACAAG | CTCGTTGTTGTGCAGTTGCA |
| mWnt7a | TGGACCACACTGCCACAGTT | GACGGCCTCGTTGTATTTGTC |
| mWnt7b | GCAGCTACCAGAAGCCTATGGA | TCGCAGTAGTTGGGCGACTT |
| mWnt8a | TCTGCCTGGTCAGTGAACAACT | TGGCGGTGTAGGTCAGATAGG |
| mWnt8b | TGCCCCGAGAGAGCTTTACA | CAAATGCTGTCTCCCGGTTAG |
| mWnt9a | CGTGGGTGTGAAGGTGATAAAG | ACACACCATGGCATTTGCAA |
| mWnt9b | GGGACCTGGTCTACATGGAAGA | GTGCCCGGAGAGTACTTGCT |
| mWnt10a | CGGAACAAAGTCCCCTACGA | CGAAAGCACTCTCTCGAAAACC |
| mWnt10b | ATGCGGATCCACAACAACAG | GCACTTCCGCTTCAGGTTTTC |
| mWnt11 | CTCCCCTGACTTCTGCATGAA | CTTGTTGCACTGCCTGTCTTG |
| mWnt16 | TCAGGAGTGCAGAAGCCAGTT | AAGTGGTAGTGGCGACCATACA |
| mDkk1 | TGCCTCCGATCATCAGACTGT | CTTGGACCAGAAGTGTCTTGCA |
| mSost | ACTTGTGCACGCTGCCTTCT | TGACCTCTGTGGCATCATTCC |
| mβ-catenin | GATGCAGCGACTAAGCAGGAA | GGAACCCAGAAGCTGCACTAGA |
| mLef1 | CTGGCAAGGTCAGCCTGTTTA | GTGTCGCCTGACAGTGAGGAT |
| mC-myc | CAGCTCGCCCAAATCCTGTA | CGAGTCCGAGGAAGGAGAGA |
| mCyclinD1 | AGAAGGAGATTGTGCCATCCA | CTCACAGACCTCCAGCATCCA |
| mCox2 | TGCAGAATTGAAAGCCCTCT | CCCCAAAGATAGCATCTGGA |
| mRunx2 | GCCGGGAATGATGAGAACTA | GGTGAAACTCTTGCCTCGTC |
| mβ-Tubulin | CTGCTCATCAGCAAGATCAGAG | GCATTATAGGGCTCCACCACAG |
| TOP FLASH ChIP | TCATGTCTGGATCCAAGCT | GCTGGAATTCGAGCTCCCA |
| Axin2 ChIP | TGCTTGCCACTGTTTGAAGTCAGC | GCCATGAACCCTTTTTGATCTTGC |
| Hdac4 ChIP | TGAAAGCACCGCTCATTCTCTGTG | GCTGCCTTAAACTTGGCATCAAAGG |
| Mmp20 ChIP | TTCCTTCTTAGTTTGCTTCACTGGAAA | CATGGTTGATTTCTTGGCTTCTGAC |
| Myc1 ChIP | TGAGAGGTGGAGAAAGAGATGTTCA | CCCCAGAGACACAAAAGGGAAGA |
| Runx2 ChIP | CGTAGTAGTACACAACGCCG | GTTTCGTGTCTGT CTTCCCC |