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. 2017 May 18;8:753. doi: 10.3389/fpls.2017.00753

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Copyright © 2017 Gao, Cui, Cao, Liu, He, Zhang and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR validation of B. elliptica-responsive miRNA target genes in L. regale (Conting 1715, Conting 39906, Conting 5555, Conting 6476, Conting 4725, and Conting 5180 represent genes encoding EF1, SODIUM/HYDROGEN EXCHANGER 6, HSP, HYPOTHETICAL PROTEIN 1, TCP2, and VESICLE-ASSOCIATED MEMBRANEPROTEIN). The amount of expression was normalized to the expression level of the CLATHRIN gene. The normalized mRNA levels at 0 h were arbitrarily set to 1. Each bar shows the mean ± SE of triplicate assays. ^* or ^** indicates a statistically significant difference as relative to the value at 0 h for each miRNAs at p < 0.05 or 0.01, respectively.