Table 1.
(A) The Number of Nucleosomes Grouped by the Initial Step of Salt-Induced Dimer Dissociation, (B) The Number of Nucleosomes Categorized by the Initial Step of Nap1-Mediated Dimer Dissociation, (C) Statistical Analysis on the Numbers of Nucleosomes Showing Dimer Displacement First in the Three Cases of Nap1-Mediated Dimer Dissociation
| (A) | ||||||
|---|---|---|---|---|---|---|
| Salt-induced dimer dissociation | ||||||
| DNA unwrapping first | Dimer displacement first | |||||
| WT | H4K16ac | H3K56ac | WT | H4K16ac | H3K56ac | |
| # of events | 160 | 117 | 69 | 9 | 8 | 4 |
| in % | 95 ± 2 | 94 ± 2 | 95 ± 3 | 5 ± 2 | 6 ± 2 | 5 ± 3 |
| (B) | ||||||
|---|---|---|---|---|---|---|
| NAP1-mediated dimer dissociation | ||||||
| DNA unwrapping first | Dimer displacement first | |||||
| WT | H4K16ac | H3K56ac | WT | H4K16ac | H3K56ac | |
| #of events | 78 | 38 | 71 | 130 | 39 | 59 |
| in % | 37 ± 3 | 49 ± 6 | 55 ± 4 | 62.5 ± 3.4 | 51 ± 6 | 45 ± 4 |
| (C) | |||
|---|---|---|---|
| NAP1-mediated dimer dissociation | |||
| Dimer displacement first | |||
| WT | H4K16ac | H3K56ac | |
| in % | 62.5 ± 3.4 | 51 ± 6 | 45 + 4 |
| Δ % from WT | – | −12 ± 7 | −18 ± 5 |
| p-value (one-tailed) | – | <0.00001 | <0.00001 |