Figure 2. Loss of Phf8 confers resilience to anxiety.

(a) Diagram of open field test. Mice are placed in an open, lit chamber and locomotion and time spent in the centre are monitored for 20 min. (b) Locomotion as quantified by sensor beam breaks within the open field chamber. (c) Time spent in the centre by Phf8 WT and KO mice. (d) Diagram of the elevated plus maze assay. Mice are placed in the centre of an elevated maze containing two open and two enclosed arms; time spent on each arm is monitored for 5 min. (e) Quantification of time spent by Phf8 WT and KO mice on each arm during the elevated plus maze assay. (f) Quantification of the fraction of time spent in the open arms over the total time for each genotype. (g) Scheme for light/dark box assay. Mice are placed in a container containing an enclosed ‘dark' half and an open ‘light' half. Time spent in either half is measured over a 5-min period. (h) Quantification of results from the Light/Dark box assay; the total time spent in the anxiogenic light box is shown. (i) Latency to first entry to the light box in the Light/Dark box assay is shown. Error bars=s.e.m. *=p<0.05, ^***=p<0.001 (Student's t-test two-Tailed).