Figure 3. Phf8 deficient mice are resilient to depression.

(a) Experimental setup for forced swim assay. Mice are placed in a platform-less beaker filled with water and subsequently monitored for 3 min. (b) Time spent immobile in forced swim assay. (c) Latency to first immobility in forced swim assay. (d) Tail-suspension assay diagram. Mice are taped, suspended by the tail and their immobility is monitored for 5 min. (e) Time immobile in a tail suspension assay. (f) Latency to first immobility for tail suspension assay (g) Experimental design of social defeat assay. Experimental mice are subject to social defeat by exposure to an aggressive mouse over a 10-day training period, then placed with a novel mouse and time spent in the interaction zone (green) versus opposite corners (red) is quantified. (h) Time spent in interaction zone for social defeat assay. Control: no social defeat during training phase, No target: no novel mouse in experimental phase, Target: novel mouse in experimental phase. (i) Time spent in corners during social defeat assay. WT control no target n=10, WT defeat no target n=9, KO control no target n=10, KO defeat no target n=10, WT control target n=10, WT defeat target n=7, KO control target n=10, KO defeat target n=9. Error bars=s.e.m. *=p<0.05, ^**=p<0.01 (Student's t-test, two-tailed).