Skip to main content
. 2017 May 9;8:15068. doi: 10.1038/ncomms15068

Figure 4. Allogeneic donor engraftment is impaired by host-Treg-depletion.

Figure 4

(a) Experimental scheme: B6 WT or B6 FTR (both H-2b) mice with or without DT treatment on day −2 and −1 were lethally irradiated and transplanted with 1 × 107 TCD BM from allogeneic BALB/c (H-2d) mice. (b) Survival of transplanted mice. Comparisons have been made between DT-untreated and DT-treated B6 WT and B6 FTR mice. The graph shows the results of three-pooled consecutive experiments (***P<0.001, log-rank test). (c) Representative histological haematoxylin and eosin stained section of BM derived from allogeneic transplanted untreated or DT-treated FTR mice 4 weeks after transplantation. (d) PB donor chimerism of total CD45+ cells over time after transplantation of DT-treated and untreated B6 WT or B6 FTR mice. Statistical analysis of the differences between FTR and DT-treated FTR at different time points are shown. (e) PB donor B-cell percentage over time after transplantation of DT treated and untreated B6 WT or B6 FTR mice. Statistical analysis of the differences between FTR and DT-treated FTR at different time points are shown. (f–h). PB donor chimerism of CD4+ T cells (f), CD8+ T cells (g), and Myeloid cells (h) over time after transplantation of DT-treated and untreated B6 WT or B6 FTR mice. Statistical analysis of the differences between FTR and DT-treated FTR at different time points are shown. Data are shown as means±s.d. ns=not significant, *P<0.05, **P<0.01, ***P<0.001, Student's t-test. (i) Survival of transplanted mice when host-Treg cells have been adoptively transferred to rescue donor BM engraftment. Comparisons have been made between DT-untreated FTR mice, DT-treated FTR mice and DT-treated FTR mice that received host-Treg cells infusion at day 0 of transplantation. Data reported is the results of two-pooled consecutive experiments. **P<0.01, log-rank test. (j,k) Total CD45+ cell donor chimerism (j) and donor B-cell percentage (k) in PB of untreated FTR mice, DT-treated FTR mice and DT-treated FTR mice that were rescue with host-Treg cells infusion at day 0 of transplantation are shown. Statistical analysis of the differences between DT-treated FTR and DT-treated FTR rescued with host-Treg cells at different time points are shown. Data are shown as means±s.d. ns=not significant, *P<0.05, **P<0.01, ***P<0.001, Student's t-test.