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. 2017 May 9;8:15186. doi: 10.1038/ncomms15186

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Composition of the bacterial community in the gut of N. vespilloides larvae and the midgut and hindgut of adult males and females was studied by TEFAP analysis of 16S rRNA gene sequences. The heat map shows the abundance of bacterial taxa (log-transformed), with warm colours indicating high abundance, cold colours indicating low abundance and black denoting absence. Taxonomy was assigned to representative sequences using the RDP classifier implemented in QIIME, and the phylogeny was generated in FastTree, based on an alignment produced using SINA and ARB. The samples were clustered based on the unweighted UniFrac distances of microbial community profiles (AD, adult; M, male; F, female; MG, midgut; HG, hindgut).