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. 2017 May 9;8:15272. doi: 10.1038/ncomms15272

Figure 3. New mitochondrial candidate proteins.

Figure 3

(a) Volcano plot as shown in Fig. 2b highlighting the 331 newly identified mitochondrial candidate proteins in black. Validated new mitochondrial candidate proteins (see b) are annotated by gene IDs as shown. All other proteins are depicted in light grey. (b) Immunofluorescence microscopy analysis of selected mitochondrial candidate proteins in T. brucei. Candidates were expressed as C-terminally tagged c-Myc fusion proteins (red) and stained using anti-Myc antibodies. Mitochondria were visualized using anti-ATOM40 serum (green), nuclear and mitochondrial DNA were stained with 4,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 10 μm. (c) Prediction of mitochondrial targeting sequences for new mitochondrial candidate proteins. Shown are the total numbers and the overlap of candidates with predicted N-terminal signal peptides according to TargetP and MitoFates. (d) Functional classification of new mitochondrial candidate proteins according to GO slim terms in the domains ‘biological process' (BP), ‘cellular component' (CC) and ‘molecular function' (MF). The number of proteins assigned to a given term is indicated. (e) Overlap of human, mouse and yeast proteins identified as homologues of the new mitochondrial candidate proteins.