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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Endocrinol. 2017 Feb 9;233(1):119–130. doi: 10.1530/JOE-16-0502

Figure 2. Expression of factors required for embryo implantation and decidualization during uterine receptivity.

Figure 2

Pseudopregnancy was in induced in Control (n=3) and GOF β-catenin (n=4) mice and uteri were analyzed on DOPP 4.5 for expression of factors important for implantation and decidualization. pERK1/2 (A) and pSTAT3 (B; P=0.004) were semi-quantified by WB in control and GOF β-catenin mice. Serum estradiol (C) and progesterone (D; P=0.057) levels were assessed by ELISA and ERα (E-G) and LIF (H) were compared by IHC and WB, respectively in control and GOF β-catenin mice. (G) IgG control for anti-ERα antibody. LIF expression (H) was normalized to β-Tubulin, and pERK1/2 (A) and pSTAT3 (B) were normalized to the respective total protein levels. Values are the mean +/− s.e.m. compared by T-test. Representative images of uterine cross-sections shown for ERα IHC. LE, luminal epithelium; GE, glandular epithelium; ST, stroma; MYO, myometrium.