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Generation of stable RhoA siRNA-transfected SPCA1 lung cancer cells and western blot analysis of RhoA protein expression. (A) Sequence analysis of recombinant plasmids and synthesized siRNA oligonucleotides. (B) RhoA siRNA-transfected SPCA1 cell lines under a fluorescence microscope (magnification, ×50). (C) Western blot analysis of RhoA protein expression. Lane 1, control cells; lane 2, RhoA siRNA-transfected cells. Rho, Ras homolog family member A; siRNA, small interfering RNA.
