Figure 5.

Protein expression levels of STAT3 and p-STAT3 in RhoA siRNA-transfected SPCA1 cells. (A) STAT3 and p-STAT3 (Tyr 705) protein expression levels were measured by western blotting and subsequent densitometry analysis (mean ± standard error; n=3). (B) Quantification of STAT3 and p-STAT3 protein expression levels. Following RhoA knockdown, the level of p-STAT3 decreased by 31.5% in RhoA siRNA-transfected cells, whereas RhoA siRNA did not significantly affect STAT3 protein levels. **P<0.01 vs. control. STAT3, signal transducer and activator of transcription 3; p-STAT3, phosphorylated-STAT3; RhoA, Ras homolog family member A; siRNA, small interfering RNA.