Figure 2.

Dose-dependent effects of BBR on the expression of lipogenesis-associated genes. 3T3-L1 adipocytes were treated with 0, 5, 10, 15 or 20 µM BBR from days 2–7 of differentiation, and cells were harvested on day 7 for analysis. The mRNA levels of (A) FAS, (B) LPL, (C) SREBP-1c, (D) PPARγ, (E) C/EBPα and (F) ACC-1 were measured by reverse transcription-quantitative polymerase chain reaction. Different letters indicate significant differences between groups (P<0.05). BBR, berberine; FAS, fatty acid synthase; LPL, lipoprotein lipase; SREBP1-c, sterol regulatory element binding protein-1c; PPARγ, peroxisome proliferator-activated receptor-γ; C/EBPα, CCAAT/enhancer-binding protein α; ACC-1, acetyl-CoA carboxylase-1.