Figure 5.

Effect of BBR on the expression of lipogenesis-associated genes over time. 3T3-L1 adipocytes were treated with 0 or 20 µM BBR commencing at day 2 until cells were harvested at days 3, 5 or 7 of differentiation. The mRNA levels of (A) FAS, (B) LPL, (C) SREBP-1c, (D) PPARγ, (E) C/EBPα and (F) ACC-1 were measured by reverse transcription-quantitative polymerase chain reaction. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated adipocytes at same day of differentiation. BBR, berberine; FAS, fatty acid synthase; LPL, lipoprotein lipase; SREBP1-c, sterol regulatory element binding protein-1c; PPARγ, peroxisome proliferator-activated receptor-γ; C/EBPα, CCAAT/enhancer-binding protein α; ACC-1, acetyl-CoA carboxylase-1.