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. 2017 Apr 12;15(6):3521–3528. doi: 10.3892/mmr.2017.6472

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Copyright: © Ge et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Synergistic effects of ZD55-TIS and SNS-032 in vivo. (A) Tumor volume was measured at different times after treatment. Data are presented as the mean ± standard deviation (n=7). **P<0.05, with comparisons indicated by lines. (B) The image presents inhibitory effect of each group on tumor growth at the last time point (day 60) when the mice were sacrificed. (C) Subcutaneous BxPC-3 tumors were collected 7 days after injection, and sections were analyzed by different methods. H&E staining showed that tumor tissues treated with the combination of ZD55-TIS and SNS-032 exhibited the strongest cell death (original magnification, ×400). Immunohistochemistry analysis demonstrated there was strong expression of TRAIL and Smac in xenografts treated with ZD55-TIS and the combined therapy group. However, the combined therapy group had a more obvious downregulation of CDK-2, −7 and −9 expression compared with SNS-032 alone. TUNEL assay revealed enhanced cell apoptosis in the group treated with ZD55TIS plus SNS-032. ZD55-TIS, ZD55-TRAIL-IETD-Smac; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; IETD, isoleucine-aspartate-threonine-glutamate; Smac, second mitochondria-derived activator of caspase; H&E, hematoxylin and eosin; CDK, cyclin-dependent kinase; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.