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. 2017 Apr 24;15(6):4084–4092. doi: 10.3892/mmr.2017.6504

Figure 2.

Figure 2.

Relative levels of Dectin-1, TLR-2 and TLR-4 (A) mRNA and (B) protein expression in THP-1 cells at 48 h following transduction with AdPU.1, as determined by RT-qPCR and western blot analyses, respectively (*P<0.05 vs. Mock group; #P<0.05 vs. Ad group). Relative levels of Dectin-1, TLR-2 and TLR-4 (C) mRNA and (D) protein expression in THP-1 cells at 48 h following transfection with siPU.1, as determined by RT-qPCR and western blot analyses, respectively (*P<0.05 vs. Mock group; #P<0.05 vs. siNC group). Dectin-1, dendritic cell-associated C-type lectin receptor-1; TLR, toll-like receptor; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Ad-PU.1, recombinant adenoviral-PU.1 vector; siPU.1, small-interfering RNA targeting PU.1; mock, untransfected/transduced cells; Ad, adenovirus-transduced cells; siNC, negative control siRNA.