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. 2017 Apr 24;15(6):4084–4092. doi: 10.3892/mmr.2017.6504

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — mRNA levels of (A) TNF-α, (B) IL-10 and (C) IL-1β in mock, Ad, or Ad-PU.1-transduced THP-1 cells at 0, 4, 8 and 12 h of exposure to resting conidia, as determined by reverse transcription-quantitative polymerase chain reaction analysis. The protein levels of (D) TNF-α and (E) IL-10 were assayed by enzyme-linked immunosorbent assay analysis. (F) Killing ability was assessed by serial dilution of cells following co-incubation with resting conidia, and quantification of the number of colony forming units. (G) Confocal laser-scanning microscopy was used to observe phagocytosis after 4 h of incubation with resting conidia. Magnification, ×63 (objective oil immersion lens). *P<0.05 vs. Mock group; ^#P<0.05 vs. Ad group. TNF, tumor necrosis factor; IL, interleukin; Mock, untransduced cells; Ad, adenovirus-transduced cells; Ad-PU.1, recombinant adenoviral-PU.1 vector.