Figure 2.
Exposure to γ-rays altered GDF-15 protein expression levels in AHH-1 cells. AHH-1 cells were irradiated with 0–10 Gy of 60Co γ-rays at a dose rate of 1 Gy/min. Cells were collected for western blot analysis 8–72 h post-irradiation. (A) Representative blots demonstrating alterations in GDF-15 protein expression levels. (B) Semi-quantification of western blot analysis results, normalized to β-actin. Relative GDF-15 protein expression levels were significantly upregulated following irradiation compared with the control group (0 Gy). Data are expressed as the mean ± standard error of the mean of three independent experiments. *P<0.05 vs. 0 Gy group. GDF, growth/differentiation factor.