Table III.

Amplification reaction conditions for bisulfate direct sequencing.

Name	Amplification reaction conditions
FHypeMR 1	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 56°C, 1 min at 72°C; 10 min at 72°C
FHypeMR 2	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54°C, 1 min at 72°C; 10 min at 72°C
FHypeMR 3	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54°C, 1 min at 72°C; 10 min at 72°C
FHypeMR 4	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54.5°C, 1 min at 72°C; 10 min at 72°C
FHypeMR 5	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 55°C, 1 min at 72°C; 10 min at 72°C
FHypoMR 1	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54.5°C, 1 min at 72°C; 10 min at 72°C
FHypoMR 2	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 53°C, 1 min at 72°C; 10 min at 72°C
FHypoMR 3	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54°C, 1 min at 72°C; 10 min at 72°C
FHypoMR 4	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54.5°C, 1 min at 72°C; 10 min at 72°C
FHypoMR 5	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 54°C, 1 min at 72°C; 10 min at 72°C
FHypoMR 6	4 min at 98°C; 30 cycles for 45 sec at 94°C, 45 sec at 53°C, 1 min at 72°C; 10 min at 72°C

FHypeMR, fetus hypermethylation regions; FHypoMR, fetus hypomethylation regions.