Figure 3.

Disruption of Taxol-induced MT polymerization decreases GTP-RhoA protein expression levels and reverses cell cycle arrest. Cells pre-treated with the MT depolymerizing agent, Col, (A) exhibited reduced MT bundling and (B) GTP-RhoA protein expression levels, which was determined by western blotting and (C) flow cytometry. (D) Taxol-induced cell cycle arrest was reversed following col treatment. Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 and **P<0.01 vs. control; ^#P<0.05 vs. Taxol. Scale bar=10 µm. MT, microtubule; GTP, guanosine triphosphate; RhoA, Ras homology gene family, member A; Col, colchicine; MF, actin/myosin microfilaments.