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. 2017 May 10;8:15293. doi: 10.1038/ncomms15293

Figure 4. The Munc13-1/RIM heterodimer complex is optimal for docking.

Figure 4

(a) Scheme corresponding to the structure of the full-length Munc13-1 (WT), homodimerization mutant (K32E), heterodimerization mutant (E128K, E137K) and homo/heterodimerization mutant (K32E, E128K, E137K) used for rescues in Munc13-1/2 DKO hippocampal neurons. (b) Representative electron micrographs of synapses showing docked synaptic vesicles (indicated by red arrows) from Munc13-1/2 DKO hippocampal cultures and DKO rescued with the respective Munc13-1 WT and mutants indicated above. Scale bar, 100 nm. (c) Plot of number of docked SVs. (d) Plot of AZ length in nm. Numbers in bar graphs represent the n values for each group. Significances and P values were determined by one-way analysis of variance (ANOVA) with Kruskal–Wallis test followed by Dunn's post test. Values indicate mean±s.e.m.; **P<0.01; ***P<0.001.