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. 2017 Apr 27;15(6):4132–4138. doi: 10.3892/mmr.2017.6517

Figure 1.

Figure 1.

Purification and identification of recombinant PPI protein. (A) SDS-PAGE analysis of PETppi transformed E.coli BL21 (DE3) and purified recombinant PPI. Lane 1, protein marker; lane 2, crude extract of pETppi transformed E. coli BL21 (DE3) prior to induction; lane 3, crude extract of pETppi transformed E. coli BL21 (DE3) after induction with 1 mM IPTG for 4 h; lane 4, purified recombinant PPI protein. (B) ESI-Q-TOF MS of purified recombinant PPI protein. The partial sequences of 4 peaks from the recombinant PPI were identified by MS/MS (Arrow).