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PI3K, Akt, mTOR gene primer sequences and amplification fragment length of the product.
| Genes | Sequence (5′→3′) | Product size (bp) |
|---|---|---|
| TGF-β1 | U: GCCCTGGACACCAACTATTG | 1,724 |
| D: CGTGTCCAGGCTCCAAATG | ||
| PI3K | U: GATTCTCAGCAGCCAGCTCTGAT | 863 |
| D: GCAGGCTGTCGTTCATTCCAT | ||
| Akt | U: GACAACCGCCATCCAGAC | 851 |
| D: CCAGGGACACCTCCATCTC | ||
| mTOR | U: ACTCGCTTCTATGACCAACTGA | 494 |
| D: TTTCCATGACAACTGGGTCATTG |
U, upstream; D, downstream; PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; mTOR, mammalian target of rapamycin.
