Figure 2.

Changes in the content of total and phosphorylated GSK3β in the hippocampus of aged mice treated with tibolone (TIB).

Western blots for total (A) and phosphorylated (Ser9) (B) GSK3β content in the hippocampus of aged mice. GAPDH was used to correct differences of total loaded protein. Proteins detected by western blot analysis from the hippocampus of mice treated with TIB were quantified by densitometric analysis and corrected using GAPDH protein content data. (C) Ratio of phosphorylated (Ser9) GSK3β to GSK3β. Results are expressed as the mean ± SE of six individual experiments. *P < 0.05, vs. vehicle (Veh).