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. 2017 Apr;12(4):614–622. doi: 10.4103/1673-5374.205101

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Copyright: © Neural Regeneration Research

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Biocompatibility of collagen-chitosan (CG-CS) composite scaffolds.

(A) Co-culture of neural stem cells (NSCs) and scaffolds was observed by optical microscopy. (B) Neurites extending from cell bodies to the scaffold can be observed. Arrows in A and B indicate NSCs. (C) Proliferation of NSCs on collagen-chitosan composite scaffolds and culture medium (control) over time as detected by Cell Counting Kit-8 staining. (D) Electron microscopy shows NSCs adhered to the scaffold surface and infiltrated into inner pores. (E) Higher magnification of the rectangular area in D. Scale bars: 200 μm in A and B, 50 μm in D, 10 μm in E. (F) Percentage of scaffold biodegradation increased gradually with time. d: Day(s); w: week(s).