Figure 5.

Effect of Ca²⁺ deprivation on Schwann cell death in cultured sciatic nerve segments.

(A) Effect of EGTA treatment on the number of PI positive nuclei in the cultured sciatic nerve segment. (B, C) The images illustrate PI labeled nuclei (red) in the rat sciatic nerve segments cultured for 48 hours in medium without (B) and with EGTA (C). Values are expressed as the mean ± SEM (two-tailed t-test). ***P < 0.001. n = 4. EGTA: Ethylene glycol tetra-acetic acid; PI: propidium iodid; h: hours.