Fig. 2.

a Dorsal view of adult D. reticulatus ticks collected from a site in Harlow. Inset shows an enlarged image of the first male tick showing the distinctive ornamentation of the scutum. b Gel image showing Babesia canis mitochondrial amplicon products in D. reticulatus ticks collected in Harlow (Essex). The lane order is: 50 bp ladder (M), Female tick 1 (1), Female tick 2 (2), Female tick 3 (3), Female tick 4 (4), Female tick 5 (5), Female tick 6 (6), Female tick 7 (7), Female tick 8 (8), Male tick 1 (9), Male tick 2 (10), Male tick 3 (11), Male tick 4 (12), Male tick 5 (13), Male tick 6 (14), Male tick 7 (15), Male tick 8 (16), Male tick 9 n, 50 bp ladder (M), negative control (Neg), Theileria annulata as positive control (Pos)