Fig 9. Binding of D₁/D₅ specific ligand [³H]SCH23390 to hippocampal membranes of wt and Wfs1 knockout mice.

Comparison of specific binding of radioligand [³H]SCH23390 to hippocampal membranes of wt and Wfs1 knockout mice. (A) Binding curve of [³H]SCH23390 binding to pooled samples of wt (triangle) and Wfs1 knockout (circle) mice. The membrane suspensions (3 mg/well) were incubated with different concentrations of [³H]SCH23390 for 60 min and bound radioactivity was measured. Data are presented as mean ± SEM from experiments (n = 3) performed in duplicates. (B) The level of [³H]SCH23390 binding sites of individual wt and Wfs1 knockout mice determined in hippocampal membrane suspensions (6.7 mg/ml.) incubated with 4 nM radioligand. Data presented as mean ± SEM of all the mice tested. *P < 0.05. Data of individual mice are presented in S1 Table.