Figure 6.

Induction of chondrogenic differentiation. Proteoglycan synthesis was determined in parallel cultures that: (a) Dose dependent effects of rhTGF-β1 on proteoglycan synthesis; Treatment with the indicated concentrations of dpIhh (b) or PTHrP(1-34) (c) and in presence or absence of 1 ng/ml of rhTGF-β1 for 7 days. During the last 6 h, cultures were labeled with [³⁵S]-sulfate and incorporation into proteoglycan was measured. Values represent means ± SEM from 3 individual experiments with * p < 0.05, rhTGF-β1 (a), or dpIhh (b) or PTHrP(1-34) (c) treatment vs control at each concentration point by Student's t-test.