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The principe of LASE microscopy (a) Laser particles excited by a tightly focused optical pump beam. (b) Output energy from a laser particle as a function of pump intensity. The laser output increases steeply at pump energy above the threshold (Pth). (c) The PSF of laser emission (red line) in comparison to the traditional diffraction-limited PSF of fluorescence detection (green line). Both the resolution and signal-to-background contrast is superior in LASE microscopy.
